Free Download | Comprehensive Enzyme Kinetics

This textbook for  advanced  courses in  enzyme chemistry  and enzyme kinetics covers the field of steady state enzyme kinetics from the basic principles inherent in the Michaelis Menten equation to the expressions that describe the multi-substrate enzyme reactions. The purpose of this book is to provide a simple but comprehensive framework for the study of enzymes with the aid of kinetic studies of enzyme-catalyzed reactions. The aim of enzyme kinetics is twofold: to study the kinetic mechanism of enzyme reactions, and to study the chemical mechanism of action  enzymes. By the kinetic mechanism of enzyme reactions, we understand: (a) the kinetic mechanism, which is a qualitative description of the order of substrate combination and product release from the enzyme, and (b) the determination of ratelimiting steps from quantitative analysis of the kinetic mechanism. By chemical mechanism of action of  enzyme, we understand: (a) the identification of any intermediates, (b) the identification of any groups on the enzyme acting as acid base catalysts, (c) the roles of any cofactors, and (d) the nature of the transition state for the chemical reaction catalyzed by enzyme.